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Influence of Hydrocortisone on Chick Embryo Retina Development
Author(s) -
Vento Renza,
D'Ancona Giuseppina,
Giuliano Michela,
Taibi Gennaro,
Tesoriere Giovanni
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb05725.x
Subject(s) - endocrinology , medicine , embryo , in ovo , cortisone , retina , adrenocorticotropic hormone , thymidine kinase , retinal , biology , thymidine , hydrocortisone , hormone , chemistry , biochemistry , microbiology and biotechnology , dna , immunology , herpes simplex virus , virus , neuroscience
Treatment of chick embryos in ovo with hydro‐cortisone‐21‐phosphate (a single dose of 150 μg) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65–70%) in 8–10‐day‐old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 μg daily for 2 days) also produced an age‐dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 μg of metopir‐one, a compound that prevents the 11β‐hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.