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Modulation of Bradykinin‐Induced Inositol Trisphosphate Release in a Novel Neuroblastoma X Dorsal Root Ganglion Sensory Neuron Cell Line (F‐11)
Author(s) -
Francel Paul C.,
Miller Richard J.,
Dawson Glyn
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb05712.x
Subject(s) - dorsal root ganglion , sensory system , neuron , inositol , sensory neuron , bradykinin , neuroscience , ganglion , dorsum , chemistry , biology , anatomy , receptor , biochemistry
In the mouse neuroblastoma X dorsal root ganglion hybrid cell line F‐l 1, bradykinin receptor stimulation induced the release of inositol‐1,4,5‐trisphosphate (IP 3 ) and inositol‐1,4‐bisphosphate (IP 2 ). Maximal stimulation of [2‐ 3 H]IP 3 and [2‐ 3 H]IP 2 release by bradykinin in the absence of LiCl occurred at 7 (or less) and 15 s, respectively, with average levels of 5.7‐ (IP 3 ) and 3.4‐ (IP 2 ) fold of control values. The EC50 for bradykinin was 33 ± 5 nM . IP 3 and IP 2 concentrations returned to basal levels ∼ 1 min after bradykinin addition. Bradykinin‐induced IP 3 release was blocked by several novel bradykinin analogues. In particular, [D‐Arg 0 ]‐Hyp 3 ‐Thi 58 ‐[D‐Phe 7 ]‐bradykinin [Hyp, hydroxypro‐line; Thi, β‐(2‐thienyl)‐L‐alanine] blocked IP 3 production in a dose‐dependent fashion. Several of these analogues alone showed little or no agonist activity. The bradykinin receptor may be coupled to phospholipase C via a GTP‐sensitive protein (Gi or Go), as preincubation for 18–20 h with pertussis toxin decreased IP 3 concentrations by 45%. Bradykinin is also known to modulate the concentrations of other second messengers in neurons, increasing the concentrations of Ca 2+ , diacylglycerol (DG), and cyclic GMP and decreasing the concentration of cyclic AMP. These second messengers modulated bradykinin‐dependent IP 3 release to varying degrees. A23187, a Ca 2+ ionophore, produced a 37% decrease in IP 3 concentration. 12‐ O ‐Tetradecanoylphorbol‐13‐ace‐tate, which mimics the effects of DG and activates protein kinase C, inhibited IP 3 release by 80%. Dibutyryl cyclic GMP produced little or no inhibition of IP 3 . [D‐Ala 2 ,D‐Leu 5 ]Enkephalin (DADLE), an opioid peptide that decreases cyclic AMP concentrations, likewise had no effect. However, elevation of cyclic AMP concentrations by pros‐taglandins I 2 or E 2 or forskolin inhibited IP 3 formation in a dose‐dependent fashion. This inhibition was reduced by DADLE in a naloxone‐reversible manner, a result suggesting that the inhibition is a cyclic AMP‐mediated effect. These results show that (a) bradykinin acutely stimulates IP 3 release from F‐11 cells in a transient fashion, (b) bradykinin‐induced IP 3 release may be subject to negative feedback control mediated through protein kinase C, (c) IP 3 release is partially inhibited by pertussis toxin and by increases in cyclic AMP content, and (d) inhibition of bradykinin‐induced IP 3 release can be produced by several novel bradykinin analogues.