Premium
Hydroxyindole‐ O ‐Methyltransferase in Y‐79 Human Retinoblastoma Cells: Effect of Cell Attachment
Author(s) -
Kyritsis Athanassios P.,
Wiechmann Allan F.,
Bok Dean,
Chader Gerald J.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb05709.x
Subject(s) - retinoblastoma , enzyme , polyacrylamide gel electrophoresis , cell culture , chemistry , methyltransferase , microbiology and biotechnology , immunofluorescence , substrate (aquarium) , cell , suspension culture , biochemistry , biology , antibody , dna , methylation , genetics , gene , ecology
Hydroxyindole‐ O ‐methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y‐79 human retinoblastoma cell line. Using elec‐troblot immunolabellings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dode‐cyl sulfate‐polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.