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Biogenic Aldehydes in Brain: On Their Preparation and Reactions with Rat Brain Tissue
Author(s) -
Nilsson Goran E.,
Tottmar Olof
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb05702.x
Subject(s) - brain tissue , brain chemistry , chemistry , neuroscience , biology , endocrinology
When 1 m M serotonin, dopamine, or norepinephrine was incubated with a monoamine oxidase preparation (mitochondrial membranes) in the presence of 4 m M sodium bisulfite, 85–95% of the amines were oxidized to the corresponding aldehydes. In the absence of bisulfite, the recoveries were only ∼30%, and dark colored products were formed during the incubations. The aldehydes derived from tyramine, octopamine, methoxytyramine, and normetanephrine were also prepared by the use of this method. The bisulfite‐aldehyde compounds were stable during storage at −20°C. Bisulfite‐free aldehyde solutions were made by diethylether extraction. When the aldehydes derived from do pamine or serotonin were incubated with rat brain homogenates, they were found to disappear in an aldehyde dehydrogenase‐ and aldehyde reductase‐independent manner. The disappearance of the latter aldehyde was more pronounced, and the results indicated that this aldehyde may react with both proteins and phospholipids.