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A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N ‐Acetyltransferase
Author(s) -
Namboodiri M. A. A.,
Brownstein M. J.,
Voisin Pierre,
Weller Joan L.,
Klein David C.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb04132.x
Subject(s) - simple (philosophy) , acetyltransferase , chemistry , chromatography , biology , biochemistry , philosophy , epistemology , acetylation , gene
A two‐step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N ‐acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500‐fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N ‐acetyl‐transferase.