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Retrograde Transport of Endogenous Nerve Growth Factor in Superior Cervical Ganglion of Adult Rats
Author(s) -
Nagata Yutaka,
Ando Masato,
Takahama Kazuya,
Iwata Mitsuyoshi,
Hori Shinichiro,
Kato Kanefusa
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb03429.x
Subject(s) - superior cervical ganglion , endogeny , nerve growth factor , axoplasmic transport , ganglion , cervical ganglia , neuroscience , anatomy , endocrinology , medicine , biology , chemistry , receptor
: The concentration of naturally synthesized nerve growth factor (NGF) was measured in various tissues of adult rats, using a highly sensitive two‐site enzyme immunoassay. The highest concentration was found in the superior cervical sympathetic ganglion (SCG). Transection of the postganglionic external carotid nerve (ECN) reduced the ganglionic level of NGF more than did section of the internal carotid nerve (ICN). When both the preganglionic nerve and the ECN were cut, the ganglionic NGF level decreased even more. On the other hand, when the preganglionic nerve and the ICN were both sectioned, leaving the ECN intact, endogenous NGF content in the SCG was significantly enhanced 3–9 h after operation. Bilateral extirpation of submaxillary gland produced a rapid decrease in ganglionic NGF 3–6 h after operation, and even unilateral re moval of one salivary gland caused a decrease in both ganglia, which was however much greater in the ipsi‐than iN the contralateral ganglion. Removal of the eyeballs caused a much smaller reduction in ganglionic NGF than did re moval of the glands. These results suggest that the endoge nous NGF that accumulates in the SCG is mostly synthe sized in the submaxillary gland rather than in the iris, and that it is transported to the SCG, mostly via the ipsilateral ECN.