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Effect of Exogenous Gangliosides on Amino Acid Uptake and Na + ,K + ‐ATPase Activity in Superior Cervical and Nodose Ganglia of Rats
Author(s) -
Nagata Yutaka,
Ando Masato,
Iwata Mitsuyoshi,
Hara Atsushi,
Taketomi Tamotsu
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb03415.x
Subject(s) - chemistry , atpase , medicine , biochemistry , enzyme
The effects of some gangliosides on active uptake of nonmetabolizable a‐aminoisobutyric acid (AIB) and Na + ,K + ‐ATPase and Ca 2+ ,Mg 2+ ‐ATPase activities in superior cervical ganglia (SCO) and nodose ganglia (NG) excised from adult rats were examined during aerobic incubation at 37°C for 2 h. In NG, amino acid uptake was greatly acceler‐a.ed with addition of galactosyl‐ N ‐acetylgalactosaminyl‐[ N ‐acetylneuraminyl]‐galactosylglucosyl ceramide (G M1 ) (85%) and also with N ‐acetylgalactosaminyl‐[ N ‐acetylneuraminyl]‐galactosylglucosyl ceramide (G M2 ) or [ N ‐acetylneuraminyl]‐galactosyl‐ N ‐acetylgalactosaminyl‐[ N ‐acetylneuraminyl]‐galactosylglucosyl ceramide (G D1a ) (43% each) compared with a nonaddition control at a 5 nM concentration. Under identical conditions, Na + ,K + ‐ATPase activity was strongly stimulated with GM1 (180%) and G D1a (93%), whereas Ca,Mg 2+ ‐ATPase activity showed no change. In SCG, on the other hand, AIB uptake was apparently inhibited (‐27%) by addition of GM1, with a slight decrease in Na + ,K + ‐ATPase but no change in Ca 2+ ,Mg 2+ ‐ATPase activity in the tissue. Both asialo‐G M1 , in which W‐acetylneuraminic acid is deficient, and Forssman glycolipid, which is not present in nervous tissue, failed to produce any significant increase in both SCG and NG not only in amino acid uptake, but also in Na + ,K + ‐ATPase activity. A kinetic study of active AIB uptake showed that G M1 ganglioside produced an increase in K m with no change in V max in SCG, whereas it caused a decrease in K m with a slight increase in V max in NG. Treatment of NG and SCG with neuraminidase from Vibrio cholerae , an enzyme that split off sialic acid from polysialoganglioside, leaving G M1 intact, caused little inhibition of the amino acid uptake. G M1 ‐evoked amino acid uptake increase in NG and decrease in SCG seem both to be independent of the effect of nerve growth factor (NGF). Glia maturation factor (GMF)‐induced AIB uptake was further increased by G M1 application in NG. These results suggest that exogenous brain gangliosides may modulate membrane transport of amino acid by processes somewhat different from those of NGF or GMF, with or without affecting the activity of a transport enzyme, Na + ,K + ‐ATPase.