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Enhanced Potassium‐Stimulated γ‐Aminobutyric Acid Release by Astrocytes Derived from Rats with Early Hepatogenic Encephalopathy
Author(s) -
Albrecht Jan,
Rafaowska Urszula
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb03385.x
Subject(s) - egta , potassium , incubation , chemistry , aminobutyric acid , calcium , histamine , stimulation , astrocyte , medicine , bicuculline , thioacetamide , endocrinology , biochemistry , biology , central nervous system , antagonist , receptor , organic chemistry
Bulk‐isolated astrocytes from rats with early hepatogenic encephalopathy (HE) induced with thioacetamide responded to the increase of potassium in the incubation medium from 5 m M to 75 m M with a markedly enhanced release of previously taken up [ 14 C]γ‐aminobutyric acid ([ 14 C]GABA). The process was not affected by omission of calcium and/or addition of EGTA to the incubation medium. Only a slight stimulation of GABA release by high potassium was observed in astrocytes from control rats. In contrast, histamine and histidine were vigorously released from control astrocytes in high‐potassium medium, and their release was not enhanced by HE, indicating that the observed phenomenon is specific for GABA.