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Na + ‐Dependent Transport of Taurine by Membrane Vesicles of Neuroblastoma ± Glioma Hybrid Cells
Author(s) -
Yuasa Shigeki,
Hamprecht Bernd
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02896.x
Subject(s) - taurine , vesicle , hypotaurine , valinomycin , membrane transport , membrane , ionophore , electrochemical gradient , chemistry , glioma , biophysics , biochemistry , neuroblastoma , membrane potential , biology , cell culture , amino acid , cancer research , genetics
The transport of taurine into membrane vesicles prepared from neuroblastoma × glioma hybrid cells 108CC5 was studied. A great part of the taurine uptake by the membrane preparation is due to the transport into an osmotically sensitive space of membrane vesicles. Taurine uptake by membrane vesicles is an active transport driven by the concentration gradient of Na + across the membrane (outside concentration > inside). The K m value of 36 μ M for Na + ‐dependent taurine uptake indicates a high‐affinity transport system. The rate of taurine transport by the membrane vesicles is enhanced by the K + gradient (inside concentration > outside) and the K + ionophore valinomycin. Taurine transport is inhibited by several structural analogs of taurine: hypotaurine, β‐alanine, and taurocyamine. All these results indicate that the taurine transport system of the membrane vesicles displays properties almost identical to those of intact neuroblastoma × glioma hybrid cells.