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Turnover of Brain Monoamine Oxidase Measured In Vivo by Positron Emission Tomography Using l ‐[ 11 C]Deprenyl
Author(s) -
Arnett Carroll D.,
Fowler Joanna S.,
MacGregor Robert R.,
Schlyer David J.,
Wolf Alfred P.,
Långström Bengt,
Halldin Christer
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02895.x
Subject(s) - monoamine oxidase b , monoamine oxidase , selegiline , in vivo , positron emission tomography , metabolite , chemistry , monoamine oxidase a , enzyme , specific activity , nuclear magnetic resonance , nuclear medicine , biochemistry , medicine , biology , physics , microbiology and biotechnology , disease , parkinson's disease
The distribution of carbon‐11‐labeled L‐deprenyl, an irreversible inhibitor of monoamine oxidase type B (MAO‐B), was determined in the baboon brain by positron emission tomography. The irreversible blood‐to‐brain transfer constant (influx constant, K i ) was measured using a complete metabolite‐corrected arterial plasma concentration curve. This influx constant was used as a measure of functional enzyme activity for sequential determinations of MAO‐B recovery following a single high dose of unlabeled l ‐deprenyl. The half‐life for turnover of MAO‐B was thus determined to be 30 days. Using appropriate irreversible inhibitors, this procedure should be generally useful for determining enzyme turnover rates in any organ in vivo and can be applied to some human studies as well.