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Effect of Lanthanum on the Release of Acetylcholine from the Myenteric Plexus and on Its Activation by Ouabain and Electrical Stimulation
Author(s) -
Doležal Vladimir,
Somogyi Gyorgy T.,
Bernath Sandor,
Tuček Stanislav,
Vizi E. Sylvester
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02892.x
Subject(s) - acetylcholine , ouabain , myenteric plexus , stimulation , ileum , medicine , endocrinology , lanthanum , chemistry , guinea pig , liberation , biology , biochemistry , sodium , in vitro , immunohistochemistry , inorganic chemistry , organic chemistry
The effect of lanthanum ions (La 3+ ) on the release of acetylcholine (ACh) from longitudinal muscle strips of the guinea pig ileum with the myenteric plexus attached was investigated. After an exposure of the tissue to 2 m M LaCl 3 for 18 min the rate of ACh release was increased approximately eightfold and the increased release lasted for more than 100 min. The augmented release of ACh was accompanied by enhanced synthesis. At the end of the experiments (102 min after LaCl 3 had been removed), when the release of ACh was still more than six times higher than in controls, the content of ACh was the same in La 3+ ‐treated and un treated tissues. Electrical field stimulation failed to cause a further increase in the release of ACh from La 3+ ‐pretreated preparations whereas ouabain released considerable more ACh when compared to controls. It is concluded from this difference that electrical stimulation and ouabain release ACh from different pools.