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A Micromethod for the Isolation of Large and Small Microvessels from Frozen Autopsied Human Brain
Author(s) -
Tsuji Teruyuki,
Mimori Yasuyo,
Nakamura Shigenobu,
Kameyama Masakuni
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02438.x
Subject(s) - sucrose gradient , centrifugation , chromatography , white matter , cerebral cortex , human brain , cortex (anatomy) , chemistry , isolation (microbiology) , pathology , biology , biochemistry , enzyme , medicine , neuroscience , magnetic resonance imaging , microbiology and biotechnology , radiology , endocrinology
Microvessels were isolated from autopsied human brain using a simple procedure involving disruption, sieving, and centrifugation on a sucrose density gradient. The present procedure is characterized by isolation, from frozen autopsied brain, of materials either from the cerebral cortex or white matter, and subsequent separation of the capillary fraction from the large vessel fraction. The preparation appears highly purified under phase‐contrast microscopic examination. The purity was also established by the enrichment of γ‐glutamyl transpeptidase activity and by the nearly negligible cerebroside content in the vessel fractions as compared to the brain homogenate.