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Mediation of Norepinephrine‐Stimulated Cyclic AMP Accumulation by Adrenergic Receptors in Hypothalamic and Preoptic Area Slices: Effects of Estradiol
Author(s) -
Etgen Anne M.,
Petitti Nicolas
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02430.x
Subject(s) - medicine , endocrinology , prazosin , agonist , phenylephrine , methoxamine , chemistry , norepinephrine , yohimbine , propranolol , receptor , antagonist , biology , dopamine , blood pressure
Adrenergic receptor agonists and antagonists were employed to establish (a) which receptor subtypes mediate the cyclic AMP response to norepinephrinc in hypo‐ thalamic and preoptic area slices from gonadectomized female rats and (b) which receptor subtypes might be modulated by the steroid hormone estradiol. Slice cyclic AMP levels were elevated by the β receptor agonist isoproterenol, but not by α 1 (phenylephrine, methoxamine) or α 2 (clonidine) agonists. However, the a agonist phenylephrine potentiated the effect of the β agonist isoproterenol on slice cyclic AMP accumulation. In slices from rats given no hormone treatment, the β antagonist propranolol inhibited norepinephrine‐stimulated cyclic AMP production, while the α 1 antagonist prazosin was without effect. In contrast, the cyclic AMP response to norepinephrine in slices from estradiol‐treated rats was blocked more effectively by prazosin than by propranolol Estradiol treatment also attenuated the production of cyclic AMP by the β agonist isoproterenol. The data suggest (a) that norepinephrine induction of cyclic AMP accumulation in hypothalamic and preoptic area slices is med ated by β receptors and potentiated by a receptor activation and (b) that estradiol depresses β and increases α 1 eceptor function in slices from brain regions associated with reproductive physiology.