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An Immunoglobulin M Monoclonal Antibody, Recognizing a Subset of Acetylcholinesterase Molecules from Electric Organs of Electrophorus and Torpedo , Belongs to the HNK‐1 Anti‐Carbohydrate Family
Author(s) -
Bon Suzanne,
Méflah Khaled,
Musset Frédérique,
Grassi †Jacques,
Massoulié Jean
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb02429.x
Subject(s) - electrophorus , torpedo , biology , monoclonal antibody , acetylcholinesterase , biochemistry , microbiology and biotechnology , antibody , enzyme , immunology , acetylcholine receptor , receptor
An immunoglobulin M (IgM) monoclonal antibody (mAb Elec‐39), obtained against asymmetric acetyl‐cholinesterase (AChE) from Electrophorus electric organs, also reacts with a fraction of globular AChE (amphiphilic G 2 form) from Torpedo electric organs. This antibody does not react with asymmetric AChE from Torpedo electric organs or with the enzyme from other tissues of Electrophorus or Torpedo. The corresponding epitope is removed by endoglycosidase F, showing that it is a carbohydrate. The subsets of Torpedo G 2 that react or do not react with Elec‐39 (Elec‐39 + and Elec‐39 ‐ ) differ in their electrophoretic mobility under nondenaturing conditions; the Elec‐39 + component also binds the lectins from Pisum sativum and Lens culinaris. Whereas the Elec‐39 ‐ component is present at the earliest developmental stages examined, an Elec‐39 + component becomes distinguishable only around the 70‐mm stage. Its proportion increases progressively, but later than the rapid accumulation of the total G 2 form. In immunoblots, mAt Elec‐39 recognizes a number of proteins other than AChE from various tissues of several species. The specificity of Elec‐39 resembles that of a family of anti‐carbohydrate antibodies that includes HNK‐1, L2, NC‐1, NSP‐4, as well as IgMs that occur in human neuropathies. Although some human neuropathy IgMs that recognize the myelin‐associated glycoprotein did not react with, Elec‐39 + AChE, mAbs HNK‐1, NC‐1, and NSP‐4 showed the same selectivity as Elec‐39 for Torpedo G 2 AChE, but differed in the formation of immune complexes.