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Differential Postnatal Development of Monoamine Oxidases A and B in the Blood‐Brain Barrier of the Rat
Author(s) -
Kalaria Rajesh N.,
Harik Sami I.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb01031.x
Subject(s) - pargyline , monoamine oxidase , clorgyline , tryptamine , monoamine oxidase b , endocrinology , monoamine neurotransmitter , monoamine oxidase a , chemistry , medicine , cerebral cortex , serotonin , enzyme , biochemistry , biology , receptor
We studied the monoamine metabolizing mitochondrial enzyme, monoamine oxidase (MAO), in cerebral microvessels obtained from postnatally developing rats by measuring the specific binding of [ 3 H]pargyline, an irreversible inhibitor of MAO, and the rate of oxidation of three known MAO substrates: benzylamine, 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine, and tryptamine. MAO activity increased postnatally, with the greatest increase occurring in the second week and reaching a peak at 3 weeks of age. A concomitant increase in MAO of the cerebral cortex also occurred, but was several‐fold less than that of cerebral microvessels. Using clorgyline and deprenyl, relatively specific inhibitors of MAO‐A and MAO‐B, we showed that cerebral microvessels contain both forms of MAO at all ages, but there was a major preponderance in the postnatal development of MAO‐B. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analyses of rat microvessels after [ 3 H]pargyline binding also showed two distinct bands of radioactivity at all ages. These two bands corresponded to molecular weights of ∼6.5,000 for MAO‐A and ‐60,000 for MAO‐B. SDS‐PAGE resuits of brain microvessels obtained from 1‐, 14‐, and 42‐day‐old rats confirm the differential postnatal development of MAO‐B in rat brain microvessels.