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A Thermodynamic Study of 5‐[ 3 H]Hydroxytryptamine Binding to Human Cortex Membranes
Author(s) -
Todd Richard D.,
Babinski Joseph
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb01017.x
Subject(s) - membrane , chemistry , spiperone , serotonin , kinetic energy , binding site , biophysics , cortex (anatomy) , kinetics , thermodynamics , biochemistry , biology , receptor , neuroscience , physics , quantum mechanics
Kinetic and equilibrium measurements of [ 3 H]‐serotonin (5‐hydroxytryptamine) binding to human frontal cortex membranes have been made between 4 and 30°C. The effects of spiperone and ascorbate on binding have also been determined. Under the conditions used, binding was saturable and reversible. Affinity constants derived from kinetic and equilibrium data were comparable. Serotonin binding to several sites had substantial enthalpic as well as entropic components.