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[ 3 H]Norepinephrine Release from Hippocampal Slices Is an In Vitro Biochemical Tool for Investigating the Pharmacological Properties of Excitatory Amino Acid Receptors
Author(s) -
Vezzani Annamaria,
Wu HuiQiu,
Samanin Rosario
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb01011.x
Subject(s) - quinolinic acid , quisqualic acid , kainic acid , excitatory postsynaptic potential , nmda receptor , chemistry , glutamate receptor , 2 amino 5 phosphonovalerate , amino acid , glycine , hippocampal formation , excitatory amino acid antagonists , pharmacology , kainate receptor , receptor , ampa receptor , biochemistry , endocrinology , biology , tryptophan
[ 3 H]Norepinephrine ([ 3 H]NE) efflux from preloaded rat Hippocampal slices was increased in a dose‐dependent manner by excitatory amino acids, with the following order of potencies: N ‐methyl‐D‐aspartate (NMDA) < kainic acid (KA) < L‐glutamate ≥ D,L‐homocysteate < L‐aspartate < quinolinic acid < quisqualic acid. The effect of the excitatory amino acids was blocked by physiological concentrations of Mg 2+ , with the exception of KA. D.,L‐2‐Amino‐7‐phosphonoheptanoic acid dose‐dependently inhibited the NMDA effect (ID 50 = 69 μ M ), whereas at 1 m M it was ineffective versus KA. The release of [ 3 H]‐NE induced by quinolinic acid was blocked by 0.1 m M D,L‐2‐amino‐7‐phosphonoheptanoic acid. γ‐D‐Glutamyl‐glycine dose‐dependently inhibited the KA effect with an ID 50 of 1.15 m M. Tetrodotoxin (2 μ M ) reduced by 40 and 20% the NMDA and KA effects, respectively. The data indicate that [ 3 H]NE release from hippocampal slices can be used as a biochemical marker for pharmacological investigations of excitatory amino acid receptors and their putative agonists and antagonists.