Premium
A Rapid HPLC Method to Separate the Triplet Proteins of Neurofilament
Author(s) -
Karlsson J.E.,
Rosengren L. E.,
Haglid K. G.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb01002.x
Subject(s) - neurofilament , chemistry , peptide , kilodalton , high performance liquid chromatography , chromatography , biochemistry , biology , immunohistochemistry , immunology , gene
In this article a fast HPLC technique to separate the individual neurofilament proteins is described. Highly pure fractions of the three neurofilament proteins can be obtained. As much as 50 mg of each neurofilament poly‐peptide can be separated from a crude neurofilament protein preparation in one step in less than 2 h. The short separation time is of importance in minimizing degradation, especially of the 150‐kilodalton neurofilament poly‐peptide.