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Ontogenetic Profile of the Adenosine Uptake Sites in Rat Forebrain
Author(s) -
Morgan P. F.,
Montgomery P.,
Marangos P. J.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb00972.x
Subject(s) - forebrain , ontogeny , synaptogenesis , adenosine , medicine , endocrinology , receptor , biology , adenosine receptor , chemistry , binding site , microbiology and biotechnology , biochemistry , central nervous system , agonist
The ontogenesis of rat forebrain adenosine uptake sites labelled by [ 3 H]nitrobenzylthioinosine ([ 3 H]NBI) was determined and compared to that of rat forebrain adenosine receptors labelled by N 6 ‐cyclohexyl[ 3 H]adenosine ([ 3 H]‐CHA). [ 3 H]NBI binding is highly invariant with similar levels of [ 3 H]NBI binding sites from embryonic day 19 to day 30 postpartum. Scatchard and Hill analyses reveal the binding of [ 3 H]NBI in 6‐day‐old tissue to be indistinguishable from such binding in 30‐day‐old tissue. In contrast, [ 3 H]‐CHA binding is highly variant. [ 3 H]CHA binding develops slowly but steadily from about embryonic day 19, with adult binding levels being achieved at around 25 days postpartum. The ontogenetic profile of [ 3 H]CHA appears to coincide with synaptogenesis whereas that of [ 3 H]NBI does not.