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Effect of Decreased Oxygen on In Vitro Release of Endogenous 3,4‐Dihydroxyphenylethylamine from Mouse Striatum
Author(s) -
Freeman Gary B.,
Gibson Gary E.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13109.x
Subject(s) - endogeny , striatum , in vitro , chemistry , oxygen , neuroscience , biochemistry , biophysics , microbiology and biotechnology , pharmacology , biology , dopamine , organic chemistry
The effects of hypoxia on release of endogenous 3,4‐dihydroxyphenylethylamine (DA, dopamine) were investigated in mouse striatal slices. Following a 60‐min pre‐incubation, potassium increased DA release 12 times between zero and 1 min. By 10 min, uptake processes exceeded release and DA levels in the media decreased. Hypoxia (low oxygen) and anoxia (no oxygen) increased DA in the media by 120 and 205%, respectively, but did not alter dihydroxyphenylacetic acid concentrations. Under similar conditions, anoxia increased [ 3 H]DA uptake eightfold. For the uptake studies, the amount of DA added to the media was critical; in the presence of high concentrations of DA, anoxia reduced reuptake. Regardless of exogenous DA, hypoxia and anoxia increased extracellular DA, which may play a role in ischemic cell damage.