Premium
Production and Characterization of Monoclonal Antibodies Against Myelin‐Associated Glycoprotein
Author(s) -
Nishizawa Masatoyo,
Tanaka Masami,
Inuzuka Takashi,
Tanaka Keiko,
Baba Hiroko,
Miyatani Nobuyuki,
Sato Shuzo,
Miyatake Tadashi
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13104.x
Subject(s) - myelin associated glycoprotein , antibody , myelin , monoclonal antibody , glycoprotein , immunostaining , biology , peripheral blood mononuclear cell , microbiology and biotechnology , immunohistochemistry , chemistry , immunology , in vitro , biochemistry , central nervous system , endocrinology
Monoclonal antibodies against myelin‐associated glycoprotein were generated by fusing mouse myeloma cells with spleen lymphocytes from BALB/c mice immunized with human myelin‐associated glycoprotein purified from CNS myelin. Three groups of antibodies were identified: IgG antibodies recognizing the polypeptide moiety and IgG and IgM antibodies recognizing the carbohydrate moiety of the intact molecule. Properties of these antibodies were examined with sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and the immunostaining technique using human CNS and peripheral nerve myelin, and ganglioside fractions isolated from human brain and peripheral nerve, and with immunohistochemical staining of human peripheral nerves. Part of human peripheral blood mononuclear cells was stained with the antibodies against the carbohydrate moiety, but not with IgG antibodies recognizing the polypeptide moiety. Natural killer activity was partially reduced after treatment of human peripheral blood lymphocytes with an IgM antibody and complement in vitro. The possibility that anti‐myelin‐associated glycoprotein antibodies might play a role in the pathogenesis of demyelinating diseases through modification of natural killer activity is discussed.