Premium
Immunochemical and Immunohistochemical Localization of Parvalbumin in Rat Nervous Tissues
Author(s) -
Endo Toyoshi,
Takazawa Kazunaga,
Kobayashi Shigeru,
Onaya Toshimasa
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13055.x
Subject(s) - parvalbumin , globus pallidus , biology , cerebellum , lateral lemniscus , population , nucleus , central nervous system , neuroscience , microbiology and biotechnology , inferior colliculus , basal ganglia , demography , sociology
The contents of parvalbumin in various nervous tissues of the rat were measured by radioimmunoassay (RIA) and its cellular distribution was immuno‐histochemically examined by peroxidase‐antiperoxidase methods. The antibody, raised in rabbits using rat skeletal muscle parvalbumin, did not cross‐react with other Ca 2+ ‐binding proteins such as calmodulin or S‐100 proteins. The RIA demonstrated the wide distribution of the antigen, with very high levels in the cerebellum (3.217 ± 519 ng/mg protein). The immunohistochemical description by Celio and Heizmann [ Nature 293, 300‐302 (1981)] was confirmed concerning the existence of the antigen in Purkinje cells of the cerebellum; nonpyramidal neurons of the cerebral cortex; and medium‐sized cells of the olfactory bulb, hippocampus, and reticular nucleus of the thalamus. In addition to these neurons, we found the parvalbumin‐like immunoreactivity in the large neurons of the superior vestibular nucleus and the neurons of the medial superior olive nucleus. In the γ‐aminobutyric acid (GABA)‐containing nuclei such as substantia nigra, caudatoputamen, and globus pallidus. parvalbumin‐positive cells and fibers were rare. In the medial lemniscus of the midbrain which contains no GABA, parvalbumin‐immunoreactive fibers were prominent. The possibility was discussed that parvalbumin exists in a specific population of neurons that differ from those containing GABA.