Premium
Identification of a Synaptic Vesicle Antigen (M r 86,000) Conserved Between Torpedo and Rat
Author(s) -
Walker John H.,
Kristjansson Gunnar Ingi,
Stadler Herbert
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13053.x
Subject(s) - torpedo , synaptic vesicle , antigen , biology , vesicle , cerebellum , synapse , neuron , cholinergic , microbiology and biotechnology , neuroscience , biochemistry , immunology , membrane , acetylcholine receptor , receptor
Abstract: Antisera were raised in guinea pigs to synaptic vesicles purified from the electric organ of Torpedo marmorata . In cholinergic nerve terminals from Torpedo the major antigens identified had M r 300,000‐150,000, 86,000, and 18,000. The M r 86,000 antigen was conserved between Torpedo and rat, where it is neuron‐specific and concentrated in nerve terminals. When rat brain synaptosomes are subfractionated the antigen is associated with synaptic vesicles. The antigen is not found in the cytoskeleton and in the vesicle‐free cytosol. Immunohistochemical localization of the antigen in rat shows it to be associated with synapses in diaphragm, cerebellum, hippocampus, and cerebral cortex. The staining pattern of the antigen indicates that the antigen is not cholinergicspecific. The function of the M r 86,000 antigen remains to be identified.