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Effects of Cell Division, Cell Density, and Cyclic Nucleotides on Choline Acetyltransferase Activity in a Cholinergic Neuroblastoma Cell Line (S‐20Y)
Author(s) -
Kirshner Judith A.,
Markelonis George J.,
Max Stephen R.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13045.x
Subject(s) - choline acetyltransferase , forskolin , cycloheximide , intracellular , cell division , cell culture , cholinergic , biology , medicine , cell , endocrinology , cell growth , microbiology and biotechnology , biochemistry , stimulation , genetics
We investigated the effects of a number of experimental perturbations on choline acetyltransferase (ChAT) in a cholinergic mouse neuroblastoma cell line (S‐20Y). ChAT specific activity increased by 4.5‐fold during growth, suggesting that enzyme activity is dependent on increased cell density. This was confirmed by assessing enzyme activity at differential initial seeding densities. ChAT activity was also markedly enhanced by 1 mM di‐butyryl cyclic‐3′,5′‐AMP (dBcAMP), an effect that was blocked by cycloheximide. Confirmation of the dBcAMP effect was achieved with forskolin, a compound known to enhance intracellular cyclic AMP; forskolin (100 μ M ) caused a significant increase in ChAT activity. After a 20‐h latent interval ChAT activity was also enhanced significantly by cytosine arabinoside. The common element in these diverse effects on ChAT activity may be cessation of cell division, although cell‐cell interactions at the level of the cell membrane may also be important in the control of ChAT in S‐20Y.