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Changes in Regional Blood–Brain Transfer of l ‐Leucine Elicited by Arginine‐Vasopressin
Author(s) -
Brust Peter
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb13000.x
Subject(s) - vasopressin , arginine , endocrinology , medicine , chemistry , cerebral blood flow , leucine , amino acid , blood flow , biology , biochemistry
Arginine‐vasopressin (AVP), injected into the carotid artery in physiological concentration together with L‐leucine, changed kinetic constants of the blood—brain barrier (BBB) transport of this neutral amino acid without changing the cerebral blood flow (CBF). The maximum velocity of transport (V max ), the half‐saturation constant (K m ), the nonsaturable transport constant (K D ), and CBF were estimated in nine brain regions of male Wistar rats anesthetized with ether. In cerebral hemisphere, V max decreased from 21 nmol min −1 . g −1 (control) to 7.6 nmol. min −1 . g −1 (AVP). K m decreased from 0.11 to 0.029 mM. Regional differences of the kinetic constants were found in controls as well as in AVP‐treated animals. In all regions, the calculated constants V max and K m of animals coinjected with AVP were significantly decreased when compared to controls. A direct or indirect interaction of AVP with the transport system of large neutral amino acids is suggested.