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H 2 Histamine Receptors on the Epithelial Cells of Choroid Plexus
Author(s) -
Crook Richard B.,
Farber Michael B.,
Prusiner Stanley B.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12994.x
Subject(s) - choroid plexus , histamine , metiamide , endocrinology , medicine , agonist , receptor , histamine receptor , chemistry , biology , histamine h2 receptor , antagonist , central nervous system
A major site of cerebrospinal fluid production in vertebrates is the choroid plexus. The epithelial cells of the choroid plexus accumulate intracellular cyclic AMP in response to several effectors, including histamine. Since histamine is known to regulate fluid secretion in the stomach via H 2 histamine receptors, we asked whether H 2 receptors might also be present on epithelial cells of bovine choroid plexus. Using agonists and antagonists of histamine, we show that an agonist and antagonist pair specific for the H 2 subtype were clearly more effective than an H 1 agonist and antagonist pair in mimicking or inhibiting histamine stimulation of cellular cyclic AMP. Analysis by Schild plot allowed assignment of an apparent dissociation constant to the H 2 antagonist metiamide which was 34‐fold lower than that of its H 1 counterpart, diphenhydramine. These results indicate that epithelial cells of the choroid plexus possess H 2 histamine receptors.