Premium
Identification of Sodium‐Dependent, High‐Affinity Choline Uptake Sites in Rat Brain with [ 3 H]Hemicholinium‐3
Author(s) -
Manaker Scott,
Wieczorek Caroline M.,
Rainbow Thomas C.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12993.x
Subject(s) - choline acetyltransferase , cholinergic , choline , olfactory tubercle , putamen , binding site , chemistry , caudate nucleus , basal ganglia , habenula , cerebral cortex , interpeduncular nucleus , biochemistry , biology , medicine , central nervous system , neuroscience , olfactory bulb , midbrain
[ 3 H]Hemicholinium‐3 (HC‐3) was used to label sodium‐dependent, high‐affinity choline uptake sites in regions of rat brain. Autoradiography revealed a high density of [ 3 H]HC‐3 binding sites in brain regions with a high density of cholinergic terminals, such as the interpeduncular nucleus, caudate‐putamen, and olfactory tubercle. This distribution of [ 3 H]HC‐3 binding sites was in close agreement with the amounts of choline acetyltrans‐ferase in specific nuclei and subregions of rat brain. Destruction of presynaptic cholinergic projections in the cerebral cortex and the basal ganglia by injection of excitotoxins reduced [ 3 H]HC‐3 binding by 40–50%. These data indicate that sodium‐dependent [ 3 H]HC‐3 binding sites are related to the choline transport system present in cholinergic neurons.