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Effect of Phencyclidine on the Metabolism of Individual Brain Proteins
Author(s) -
Koul Omanand,
March James E.,
Sarma Raqhupathy,
Griffiths Cynthia,
Deutsch Dale G.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12991.x
Subject(s) - phencyclidine , protein metabolism , metabolism , methionine , chemistry , in vitro , cerebral cortex , endocrinology , biology , medicine , biochemistry , receptor , nmda receptor , amino acid
The effect of phencyclidine on the metabolism of a selected number of rat brain proteins was determined using two‐dimensional gel electrophoresis and quantitative fluorography. When rats were injected with phencyclidine, modulation of individual protein metabolism occurred in the pituitary and cortex. That is, a few proteins showed increased and others decreased incorporation of [ 35 S]methionine, whereas total protein metabolism was unaltered. In contrast, in vitro treatment of brain tissue with phencyclidine inhibited incorporation of ra‐diolabel into all proteins by — 50%, as shown by quantitative fluorography of individual proteins.