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Binding of Bodian's Silver and Monoclonal Antibodies to Defined Regions of Human Neurofilament Subunits: Bodian's Silver Reacts with a Highly Charged Unique Domain of Neurofilaments
Author(s) -
AutilioGambetti Lucila,
Crane Regina,
Gambetti Pierluigi
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12977.x
Subject(s) - neurofilament , monoclonal antibody , chemistry , chymotrypsin , microbiology and biotechnology , antibody , silver stain , protein subunit , cysteine , biochemistry , cleavage (geology) , biology , trypsin , enzyme , immunohistochemistry , gene , immunology , paleontology , fracture (geology)
Cleavage at cysteine and chymotrypsin digestion were applied to two human neurofilament (NF) sub‐units, low‐ and high‐molecular‐weight NF (NF‐L and NF‐H), to locate the regions reacting with Bodian's silver stain and with several monoclonal antibodies, including NF‐specific antibodies and one that recognizes all intermediate filaments (anti‐IFA). Our findings indicate that whereas anti‐IFA recognizes the highly conserved rod domain, all the NF‐specific antibodies, as well as Bodian's silver, react with the carboxy‐terminal tailpiece of NF subunits. The silver binding sites in NF‐L are located in a carboxy‐terminal 12‐Kd chymotrypsin fragment, a highly charged, unique domain of NF.