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Incorporation of Tritiated Galactose into Galactocerebroside by Cultured Rat Oligodendrocytes: Effects of Cyclic Adenosine 3′,5′‐Monophosphate Analogues
Author(s) -
Pleasure David,
Parris Judy,
Stern Janet,
Grinspan Judith,
Kim Seung U.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12963.x
Subject(s) - galactocerebroside , galactose , chemistry , adenosine , tritium , percoll , centrifugation , biochemistry , cyclic adenosine monophosphate , stereochemistry , chromatography , endocrinology , biology , oligodendrocyte , myelin , physics , receptor , nuclear physics , central nervous system
Cells dissociated from the forebrains of 21‐day‐old rats were enriched in oligodendroglia by Percoll gradient centrifugation, seeded on polylysine‐coated surfaces, and cultured in a serum‐containing medium. Incorporation by the cultures of tritium from d ‐[ 3 H]galactose into the galactosyl residue of galactocerebroside (galC) increased in an almost linear fashion for 48 h with 1–8 μCi of d ‐[ 3 H]galactose (30 mCi/μmol) per milliliter medium. Treatment for 2 days (day 1–3 after seeding) with 10 −4 M or 10 −3 M dibutyryl cyclic adenosine 3′,5′‐monophosphate (db cyclic AMP) or 10 −4 M 8‐bromo cyclic AMP stimulated galC radiolabelling. Incorporation of d ‐[ 3 H]galactose into galC during a terminal 48‐h radiolabelling period was not stimulated when the cells were continuously treated with these cyclic AMP analogues for 8 rather than 2 days.