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Peanut Agglutinin Receptor Is a Marker of Myelin in Rat Brain. Developmental Changes in Its Distribution
Author(s) -
Momoi Takashi,
Yoshida Momoi Mariko,
Kurata Takeshi
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12951.x
Subject(s) - peanut agglutinin , myelin , distribution (mathematics) , agglutinin , biology , myelin sheath , neuroscience , receptor , central nervous system , immunology , lectin , biochemistry , mathematical analysis , mathematics
Peanut agglutinin receptor, which is a glyco‐conjugate with terminal Gal(β1–3)GalNAc residues, was found to be a specific biochemical marker of the myelin in rat brain. The localization of peanut agglutinin receptor in rat brain was studied by use of fluorescein‐conjugated peanut agglutinin. In adult rat brain, receptors were found only on myelin. In contrast, the receptors in fetal brain were not found in the white matter, but only in the ependyma. Peanut agglutinin receptors were extracted from adult and fetal rat brains with 2% Triton X‐100, subjected to electrophoresis on 10% sodium dodecyl sulfate‐polyacrylamide gel, blotted onto nitrocellulose filters, and detected by their reaction with peroxidase‐conjugated peanut agglutinin. A major receptor of M r 130,000 and a minor one of M r 200,000 were found in both adult and fetal rat brains.