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Noninvasive In Vivo Demonstration of 2‐Fluoro‐2‐Deoxy‐ d ‐Glucose Metabolism Beyond the Hexokinase Reaction in Rat Brain by 19 F Nuclear Magnetic Resonance Spectroscopy
Author(s) -
Nakada Tsutomu,
Kwee Ingrid L.,
Conboy Claire B.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb12945.x
Subject(s) - in vivo , nuclear magnetic resonance spectroscopy , hexokinase , nuclear magnetic resonance , chemical shift , metabolism , chemistry , metabolite , spectroscopy , carbohydrate metabolism , glycolysis , biochemistry , stereochemistry , biology , physics , microbiology and biotechnology , quantum mechanics
The metabolism of 2‐fluoro‐2‐deoxy‐ d ‐glucose (FDG) in vivo was observed noninvasively in rat brain using “F nuclear magnetic resonance (NMR) spectroscopy following an intravenous injection of FDG (400 mg/ kg). At 3 h after infusion, four resonances with discrete chemical shifts were resolved. Chemical shift analysis of these resonances suggested the chemical identity of two of the resonances to be FDG and/or FDG‐6‐phosphate and 2‐fluoro‐2‐deoxy‐8‐phosphogluconolactone and/or 2‐fluoro‐2‐deoxy‐δ‐phosphogluconate. The chemical identities of the other two resonances remain to be elucidated. The present study indicates that the metabolism of FDG in vivo is more extensive than is previously recognized and demonstrates the feasibility of using 19 F NMR spectroscopy to follow the 19 F‐containing metabolites of FDG in vivo.