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Putative Cholinergic‐Specific Gangliosides in Guinea Pig Forebrain
Author(s) -
Ferretti Patrizia,
Borroni Edilio
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb08509.x
Subject(s) - forebrain , cholinergic , guinea pig , neuroscience , biology , cholinergic neuron , microbiology and biotechnology , endocrinology , central nervous system
The nature of the cholinergic‐specific antigen Chol‐1 recognized by an antiserum raised against Torpedo cholinergic electromotor synaptosomal plasma membranes was investigated in guinea pig forebrain to establish whether it has a gangliosidic nature in guinea pig as in Torpedo. Gangliosides extracted from guinea pig forebrain and extensively purified to eliminate peptide contaminants were effective in inhibiting the selective lysis of the cholinergic subpopulation of cortical synaptosomes induced by the antiserum. Neuraminidase, protease, alkali, and heat treatment did not impair the inhibitory activity of gangliosides. Whereas the antiserum recognized many gangliosides from Torpedo electric organ, the immunostaining of guinea pig forebrain gangliosides separated on TLC showed only two immunopositive bands migrating close to G T1b and G Q . After affinity purification on Torpedo electric organ gangliosides the activity of the antiserum in inducing complement‐mediated lysis was increased and it still recognized the two ganglioside bands on TLC. These results strongly suggest the existence of two polysialogangliosides bearing antigenic determinants specific for the cholinergic neurons.