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Effect of Nerve Growth Factor on Glucose Utilization and Nucleotide Content of Pheochromocytoma Cells (Clone PC12)
Author(s) -
Morelli Alessandro,
Grasso Marina,
Calissano Pietro
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb04512.x
Subject(s) - nerve growth factor , pheochromocytoma , clone (java method) , nucleotide , growth factor , chemistry , biology , endocrinology , medicine , microbiology and biotechnology , biochemistry , dna , gene , receptor
The effect of nerve growth factor (NGF) on the utilization and fate of uniformly labeled 14 C glucose and on the content of several pyridine and purine nucleotides has been tested in the clonal cell line PC12. After incubation for 72 h with NGF, PC12 cells exhibit a 2.7‐fold increase in glucose utilization and a 4.7‐fold increase in CO 2 release. During the same incubation period, all the nucleotides tested (NAD + , AMP, GMP, UDP‐glucose, UDP‐galactose, UDP, ADP, GDP, UTP, CTP, ATP, and GTP) underwent significant increments, varying from a minimum of 27% for ADP to a maximum of 90–120% for AMP, GMP, UDP‐glucose, and UDP‐galactose. These findings are discussed in connection with the trophic and differentiative effects of NGF in PC12 cells, which, in the presence of this factor, shifted from a neoplastic to a neu‐ronal‐like cell population.