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Biochemical Changes in Central Nervous System Membranes in Experimental Allergic Encephalomyelitis
Author(s) -
Salvati Serafina,
D'Urso Donatella,
Devirgiliis Laura Conti,
Crescenzi Giovanni Serlupi
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb02855.x
Subject(s) - myelin , encephalomyelitis , chemistry , proteolipid protein 1 , centrifugation , polyacrylamide gel electrophoresis , ovalbumin , major basic protein , biochemistry , central nervous system , gel electrophoresis , myelin basic protein , biology , immunology , endocrinology , antigen , enzyme , eosinophil , asthma
Biochemical and morphological studies of myelin subtractions were undertaken on Lewis rats during the early stage of the development of experimental allergic encephalomyelitis (EAE). Myelin subfractions, obtained by sucrose density gradient centrifugation at 10 days post‐induction, were subjected to sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and assayed for 2′:3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) activity. Aliquots were processed for electron microscopic analysis. When comparing the myelin sub‐fractions of EAE‐affected animals with those of controls, differences were observed only in the light fractions, i.e., a decrease in the specific activity of CNPase and in the percentage of basic proteins relative to the total proteins of the fraction. This decrease was also evident in the basic protein/proteolipid protein ratio which is frequently used in the literature. In addition, electron microscopic observations demonstrated strong differences in the morphology of the same fraction. These findings suggest that the light fraction is the most sensitive in the early stages of the disease and must play a key role in demyelinating processes.