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Brain Adrenoceptor Binding Sites in Mice Susceptible (DBA/2J) and Resistant (C57 Bl/6) to Audiogenic Seizures
Author(s) -
Jazrawi S. P.,
Horton R. W.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb02846.x
Subject(s) - endocrinology , medicine , abnormality , chemistry , adrenergic receptor , receptor , biology , psychiatry
We have examined if the age‐related susceptibility of DBA/2J mice to audiogenic seizures is the result of an abnormality in the number or sensitivity of brain adrenoceptors. The binding of α 1 ‐ ‐ , α 2 ‐ ‐ , and β‐adrenoceptor ligands to membranes prepared from whole brain or regions of brain of DBA/2J mice was measured at various ages, corresponding to the periods before, during, and after the maximal sensitivity to audiogenic seizures. For comparison, we have studied concurrently age‐matched C57 B1/6 mice, a strain resistant to audiogenic seizures at all ages. There was no difference in the binding of α 2 ‐ ‐ or β‐adrenoceptor ligands to whole brain membranes between the two strains of mice at any age. The maximal number of α 1 ‐ ‐ adrenoceptor binding sites was lower in whole brains of DBA/2J mice than of C57 B1/6 mice at all ages studied except 13–15 days of age. The differences were small (maximally 17%) but were statistically significant at 21–23 days of age, the time of maximal sensitivity of DBA/2J mice to audiogenic seizures. No difference between the two strains was found in the number or affinity of α 1 ‐ ‐ or α 2 ‐ ‐ adrenoceptor binding sites at any age in any of the brain regions studied. The age‐related susceptibility of DBA/2J mice to audiogenic seizures is not the result of an abnormality in number or sensitivity of α 2 ‐ ‐ or β‐adrenoceptor binding sites, but a reduced number of α 1 ‐ ‐ adrenoceptor binding sites may be involved.