Premium
Complexity of Nuclear and Polysomal RNA from Squid Optic Lobe and Gill
Author(s) -
Capano Carla Perrone,
Gioio Anthony E.,
Giuditta Antonio,
Kaplan Barry B.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb01770.x
Subject(s) - rna , biology , dna , polyadenylation , transcription (linguistics) , small nuclear rna , intron , messenger rna , gene , squid , gene expression , microbiology and biotechnology , cephalopod , non coding rna , genetics , paleontology , ecology , linguistics , philosophy
The sequence complexity of nuclear and polysomal RNA from squid optic lobe and gill was measured by RNA‐driven hybridization reactions with single‐copy [ 3 H]DNA. At saturation, brain nuclear and polysomal RNAs were complementary to 22.8 and 7.9% of the DNA probe, respectively. Assuming asymmetric transcription, the complexity of nuclear and polysomal RNA was equivalent to 2.5 × 10 8 and 8.8 × 10 7 nucleotides, respectively. Approximately 80–85% of the sequence complexity of brain total polysomal RNA was found in the polyadenylated RNA fraction. In contrast to these findings, nuclear and polysomal RNAs from gill hybridized to 9.1 and 2.9%, respectively, of the single‐copy DNA, values that were 2.5‐fold lower than those obtained in the CNS. Taken together, the results focus attention on the striking diversity of gene expression in the squid CNS and extend to the cephalopod mollusks the observation that nervous tissue expresses significantly more genetic information than other somatic tissues or organs.