Research Library

Premium Amino Acid Neurotransmitters in the CNS: Properties of Diaminobutyric Acid Transport
Author(s)
Erecińska Maria,
Troeger Melody B.,
Alston Theodore A.
Publication year1986
Publication title
journal of neurochemistry
Resource typeJournals
PublisherBlackwell Publishing Ltd
Abstract: Uptake of L‐2,4‐diaminobutyric acid (DABA), a positively charged analogue of γ‐aminobutyric acid (GABA), by a synaptosomal fraction isolated from rat brain occurred with a K m of 54 ± 12 μ M and a V max of 1.3 ± 0.2 nmol/min/mg protein. The transport of DABA was inhibited competitively by GABA whereas that of GABA was affected in the same manner by addition of DABA. The maximal accumulation of DABA ([DABA] i /[DABA] e ) was observed to increase as the second power of the transmembrane electrical potential ([K + ] i /[K + ] e ) and the first power of the sodium ion concentration gradient. These findings indicate that DABA is transported on the GABA carrier with a net charge of + 2, where one charge is provided by the cotransported Na + and the second is contributed by the amino acid itself. Since uptake of GABA, an electroneutral molecule, is accompanied by transfer of two sodium ions, the results obtained with DABA suggest that one of the sodium binding sites on the GABA transporter is in proximity to the amino acid binding site.
Subject(s)amino acid , biochemistry , biology , biophysics , chemistry , gaba transporter , gene , ion transporter , membrane , membrane transport , organic chemistry , sodium , stereochemistry , synaptosome , transporter
Language(s)English
SCImago Journal Rank1.75
H-Index229
eISSN1471-4159
pISSN0022-3042
DOI10.1111/j.1471-4159.1986.tb01761.x

Seeing content that should not be on Zendy? Contact us.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here