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Characteristics of Synthesis of Very‐Long‐Chain Saturated and Tetraenoic Fatty Acids in Swine Cerebral Microsomes
Author(s) -
Yoshida Satoshi,
Takeshita Masazumi
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb01746.x
Subject(s) - microsome , chemistry , biochemistry , enzyme
The elongation of arachidoyl‐CoA (20:0‐CoA) yielded 22:0 and 24:0 concomitantly, whereas the elongation of behenoyl‐CoA (22:0‐CoA) yielded only a negligible amount of 24:0 in adult swine cerebral microsomes. The dependence on time, pH, and the substrate concentrations were examined for the synthesis of 22:0 and 24:0 from 20:0‐CoA. A microcomputer‐aided simulation study suggested that there were two parallel pathways in the elongation of 20:0‐CoA to 22:0 and 24:0. The elongation of 22:0‐CoA could not be observed in adult swine cerebral microsomes; however, it was observed clearly in newborn swine and rat brain microsomes. A dilution experiment with the addition of cold 22:0‐CoA in the reaction of elongation of 20:0‐CoA confirmed the above suggestion that no intermediate 22:0 appeared during the synthesis of 24:0 from 20:0‐CoA. The elongation of endogenous 20:4‐CoA to 22:4 and 24:4 was examined in newborn swine cerebral microsomes, and the presence of two parallel pathways in the elongation of 20:4‐CoA to 22:4 and 24:4 similar to those involved in the elongation of 20:0‐CoA to 22:0 and 24:0 was suggested.