Premium
Release of Ethanolamine, but Not of Serine or Choline, in Rat Pontine Nuclei on Stimulation of Afferents from the Cortex, In Vivo
Author(s) -
Perschak Henry,
Wolfensberger Max,
Do Kim Quang,
Dunant Yves,
Cuénod Michel
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb01744.x
Subject(s) - stimulation , neuroscience , in vivo , choline , chemistry , cortex (anatomy) , pons , biology , biochemistry , microbiology and biotechnology
Release of ethanolamine, serine, and choline in rat pontine nuclei on electrical stimulation of afferents from the cortex was investigated using in vivo push‐pull cannula techniques. Ethanolamine was determined by using gas chromatographic techniques; serine was measured with a HPLC system; and choline was assayed with a luminescence method. Resting elution rates of ethanolamine, serine, and choline were 50.8 ± 8.4, 34.8 ± 12.6, and 1.16 ± 0.20 pmol/5 min, respectively. Stimulation of the cortico‐pontine tract evoked a highly significant 3,4‐fold increase in release of ethanolamine, whereas serine and choline release was unaffected. Reactions in membrane phospholipids are most likely involved in the stimulation‐dependent release of ethanolamine and special consideration was given to base‐exchange reactions. Alternatively, a release from intracellular, possibly synaptic stores cannot be excluded.