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Regional Differences in 5‐Hydroxytryptamine and Catecholamine Uptake in Primary Astrocyte Cultures
Author(s) -
Kimelberg H. K.,
Katz D. M.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00808.x
Subject(s) - striatum , serotonin , astrocyte , hippocampus , cerebral cortex , dopamine , endocrinology , medicine , catecholamine , desipramine , hippocampal formation , norepinephrine , cortex (anatomy) , chemistry , biology , neuroscience , central nervous system , receptor , antidepressant
The uptake of 3 H‐labelled 5‐hydroxytryptamine (5‐HT, serotonin) norepinephrine ([ 3 H]NE), and 3,4‐dihy‐droxyphenylethylamine ([ 3 H]dopamine, [ 3 H]DA) was studied in primary astrocyte cultures prepared from the cerebral cortex, corpus striatum, and hippocampal regions of neonatal rat brain. Na + ‐dependent uptake showed marked regional differences. For [ 3 H]5‐HT the magnitude of uptake was corpus striatum ≥ cerebral cortex ≥ hippocampus, whereas for [ 3 H]NE the order was hippocampus ≥ corpus striatum ≥ cerebral cortex. For [ 3 H]DA, only the hippocampal cultures showed significant Na + ‐dependent uptake. [ 3 H]5‐HT uptake was specifically inhibited by 10 ‐7 M flu‐oxetine whereas [ 3 H]NE uptake was preferentially inhibited by 10 ‐7 M desipramine. These results may reflect regional brain specialization and/or different developmental patterns of high affinity uptake of serotonin and catecholamines by astrocytes in situ.