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Thyroid Hormone Actions on a Cholinergic Neuroblastoma Cell Line (S‐20Y)
Author(s) -
Chacon Marco,
Max Stephen R.,
Kirshner Judith A.,
Tildon J. T.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00801.x
Subject(s) - choline acetyltransferase , endocrinology , medicine , thyroid hormone receptor beta , propylthiouracil , cell culture , thyroid , receptor , thyroid hormone receptor , hormone , cholinergic , chemistry , biology , hormone receptor , genetics , cancer , breast cancer
Thyroid hormone (T3) has a multiplicity of effects on the developing nervous system. We have investigated T3 action using a cholinergic neuroblastoma cell line (S‐20Y) as a model. S‐20Y contains a nuclear receptor for T3 with binding properties similar to those of other T3 target tissues. In addition, these cells can carry out 5′‐deiodination, which is necessary to produce active thyroid hormone in vivo. The enzyme involved in this process appears to be a type I deiodinase, based on its reaction kinetics and its susceptibility to inhibition by propylthiouracil. S‐20Y cells maintained in T3‐depleted medium showed decreased choline acetyltransferase (ChAT) activity. ChAT activity was restored to the control level in a dose‐dependent manner by T3 repletion. Neither cell density nor viability was influenced by the hypothyroid state. The presence of a T3 receptor and the enzyme activity for T3 production, together with an effect of T3 on ChAT activity, demonstrate that S‐20Y cells are a target for T3 action and suggest that these cells represent an excellent model system for studies of T3 effects on nervous tissues.