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Developmental Changes in Levels of Translatable mRNAs for Enolase Isozymes in Chicken Brain
Author(s) -
Tanaka M.,
Sugisaki K.,
Nakashima K.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00789.x
Subject(s) - reticulocyte , enolase , messenger rna , translation (biology) , biology , isozyme , microbiology and biotechnology , developmental profile , biochemistry , antiserum , enzyme , gene , antibody , genetics , immunohistochemistry , immunology , endocrinology
Using chicken brain mRNAs, α and γ enolase precursors were synthesized in the rabbit reticulocyte cell‐free translation system. The product proteins showed molecular weights almost identical to those of the mature subunits. The levels of translatable mRNAs for α and γ subunits were determined by the cell‐free translation system and immuno‐precipitation with specific antisera, during development of chicken brain. The level of α mRNA was high at any developmental stage of the brain. On the other hand, the γ mRNA level was very low at the early embryonic stage, and increased rapidly during development of the brain. These changes were closely correlated with those of the corresponding enzyme activities, indicating that the levels of enolase activities in developing brain were controlled primarily by the level of the translatable α and γ mRNAs.