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Binding Sites for [ 3 H]Glutamate and [ 3 H]Aspartate in Human Cerebellum
Author(s) -
Cross Alan,
Skan Wendy,
Slater Paul
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00779.x
Subject(s) - cerebellum , glutamate receptor , neuroscience , chemistry , biochemistry , biology , receptor
The binding of [ 3 H]aspartate and [ 3 H]glutamate to membranes prepared from frozen human cerebellar cortex was studied. The binding sites differed in their relative proportions, their inhibition by amino acids and analogues, and by the effects of cations. A proportion (about 30%) of [ 3 H]glutamate binding was to sites similar to those labelled by [ 3 H]aspartate. An additional component of [ 3 H]gluta‐mate binding (about 50%) was displaced by quisqualate and aL‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid, and may represent a “quisqualate‐preferring” receptor. Neither N ‐methyl‐d‐aspartic acid‐sensitive nor dl‐2‐amino‐4‐phosphonobutyric acid‐sensitive [ 3 H]glutamate binding was detected.