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Neurotensin Stimulates Inositol Phospholipid Metabolism and Calcium Mobilization in Murine Neuroblastoma Clone N1E‐115
Author(s) -
Snider R. M.,
Forray C.,
Pfenning M.,
Richelson E.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00742.x
Subject(s) - neurotensin , inositol , inositol phosphate , intracellular , neurotensin receptor , clone (java method) , phosphatidylinositol , ec50 , phospholipid , biology , receptor , endocrinology , inositol trisphosphate , medicine , biochemistry , chemistry , neuropeptide , signal transduction , in vitro , dna , membrane
Murine neuroblastoma cells (clone N1E‐115) possess neurotensin receptors that mediate cyclic GMP synthesis. Because of the hypothesized relationship between phospholipid metabolism, intracellular Ca 2+ , and cyclic GMP synthesis, we determined with these cells the effects of neurotensin on 32 P labeling of phospholipids, release of inositol phosphates, and intracellular Ca 2+ (as determined with the use of Quin‐2, a fluorescent probe sensitive to free Ca 2+ levels). Neurotensin stimulated incorporation of 32 P into phospholipids, especially phosphatidylinositol and phosphatidate. Neurotensin also stimulated the release of [ 3 H]‐inositol phosphates with an EC 50 of about 1 n M . Mean basal Ca 2+ concentration in these cells was 134 n M and this level was increased in a rapid and dose‐dependent manner by neurotensin, with an EC 50 of 4 n M . Since the EC 50 for neurotensin in stimulating cyclic GMP synthesis is 1.5 n M and the K D for binding of [ 3 H]neurotensin at 0° is 11 n M , all these different effects appear to be shared proximal consequences of neurotensin receptor activation.