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Investigation of Dopamine Content, Synthesis, and Release in the Rabbit Retina In Vitro: II. Effects of High Potassium, Adenylate Cyclase Activators, and N‐n ‐Propyl‐3‐(3‐Hydroxyphenyl) Piperidine
Author(s) -
Ofori Senyo,
Magistretti Pierre J.,
Schorderet Michel
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00741.x
Subject(s) - forskolin , adenylate kinase , endocrinology , medicine , chemistry , agonist , adenosine , cyclase , extracellular , veratridine , vasoactive intestinal peptide , tyrosine hydroxylase , dopamine , in vitro , biology , biochemistry , receptor , neuropeptide , sodium , organic chemistry , sodium channel
The modulation of 3,4‐dihydroxyphenylethylamine (dopamine, DA) synthesis and release in rabbit retina in vitro by high K + ; adenylate cyclase activators such as forskolin, 2‐chloroadenosine, vasoactive intestinal polypeptide (VIP); and the putative DA autoreceptor agonist N‐n ‐propyl‐3‐(3‐hydroxyphenyl) piperidine (3‐PPP) has been investigated. Incubation of retinas in 50 m M K + resulted in the activation of tyrosine hydroxylase (TH). Activation did not require the presence of extracellular Ca 2+ . K + 50 m M also induced a Ca 2+ ‐dependent release of DA. Forskolin 50 μ M stimulated TH but 100 μ M 2‐chloroadenosine and 650 μ M VIP did not. Individually, (±)‐3‐PPP, (‐)‐3‐PPP, and (±)‐3‐PPP reduced DA synthesis and increased its release. The effects of (+)‐3‐PPP were dose‐dependent and did not require the presence of extracellular Ca 2+ . The activation of TH induced by 50 m M K + , but not that induced by 50 μ M forskolin, was abolished by 100 μ M (±)‐3‐PPP.