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Biochemical and Immunocytochemical Evidence for a Deficiency of Normal Interfascicular Oligodendroglia in the CNS of the Dysmyelinating Mutant (md) Rat
Author(s) -
Kahn Susan,
Tansey Francine A.,
Cammer Wendy
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00720.x
Subject(s) - carbonic anhydrase , mutant , white matter , myelin , biology , carbonic anhydrase ii , biochemistry , microbiology and biotechnology , enzyme , central nervous system , neuroscience , gene , medicine , radiology , magnetic resonance imaging
Carbonic anhydrase was assayed and carbonic an‐hydrase and 5′‐nucIeotidase were localized in the CNS of myelin‐deficient mutant rats and normal littermates. The carbonic anhydrase specific activities were reduced by 61% and 29% in the mutants' forebrains and cerebella, respectively, and the total carbonic anhydrase activity in the spinal cords was reduced by 35%. Immunostained cells were found in gray matter from both normal and mutant rats, but, in the mutants, there was a marked deficiency of interfascicular oligodendrocytes in the regions that are normally occupied by white matter. It is suggested that a developmental study could indicate the step(s) at which normal differentiation of interfascicular oligodendroglia is blocked in this mutant.