Uptake and Metabolism of [ 3 H] Adenosine by Aplysia Ganglia and by Individual Neurons

[ 3 H]Adenosine was taken up and metabolized by isolated ganglia of the marine mollusc Aplysia californica . After 2 h, most of the radioactivity was recovered as metabolites, including ATP, ADP, and AMP, as well as the deaminated products, inosine, hypoxanthine, and uric acid. Little remained in the form of adenosine. These pathways were not uniformly distributed among various tissue elements. In most individual neurons, inosine and its breakdown products were the principal metabolites of [ 3 H]adenosine, whereas ATP and other nucleotides predominated in the connective tissue sheath. Endogenous levels of ATP, ADP, AMP, and adenosine in ganglia, sheath, and individual neurons were also determined using a fluorimetric‐HPLC assay. The concentrations of the nucleotides were quite uniform in sheath and among the individual neurons assayed (1–5 pmol/μg of protein); however, concentrations of adenosine were considerably higher in neurons than in the sheath.