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NAD + Glycohydrolase of the Plasma Membrane Prepared from Glial and Neuronal Cells
Author(s) -
Honma Takeshi,
Mandel Paul
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00706.x
Subject(s) - nad+ kinase , nicotinamide , membrane , enzyme , biochemistry , gtp' , chemistry , neuroglia , non competitive inhibition , microbiology and biotechnology , biology , endocrinology , central nervous system
NAD + glycohydrolase (EC 3.2.2.5) activity was detected in the plasma membrane prepared from the primary culture of rat astrocytes. The enzyme has a broad optimum pH range. From the kinetic analysis, a Michaelis constant of 91.2 μ M and a maximum velocity of 0.785 μmol/min/mg protein were obtained. ADPribose exhibited a competitive inhibition with respect to NAD. The inhibition by nicotinamide was shown to be of a noncompetitive type. ATP and GTP were found to be competitive inhibitors. NAD + glycohydrolase activity was not detected in the plasma membrane prepared from the primary culture of neuronal cells of chick embryos.