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[ 3 H]Ketanserin (Serotonin Type 2) Binding Increases in Rat Cortex Following Basal Forebrain Lesions with Ibotenic Acid
Author(s) -
Wenk Gary L.,
Engisch Kathrin L.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00688.x
Subject(s) - nucleus basalis , neocortex , hippocampus , basal forebrain , endocrinology , ketanserin , medicine , choline acetyltransferase , serotonergic , ibotenic acid , chemistry , serotonin , cholinergic , cholinergic neuron , cerebral cortex , neuroscience , biology , 5 ht receptor , central nervous system , receptor
The response of the serotonergic system following injury to the basal forebrain cholinergic system was investigated in rats. The density of 5‐hydroxytryptamine (serotonin) type 2 (S2) receptor sites in the frontal cortex and hippocampus was determined 1 week and 4 months after production of lesions by injections of ibotenic acid into the medial septum and nucleus basalis magnocellularis. One week later, the number of S2 receptor sites in the frontal neocortex, as defined by [ 3 H]ketanserin binding, was unchanged. Four months later, the number of [ 3 H]ketanserin binding sites (and B max ) was increased and high‐affinity [ 3 H]serotonin uptake was decreased in the frontal neocortex, but not in the hippocampus, relative to unlesioned controls. Choline acetyltransferase (acetyl‐CoA:choline O ‐acetyltrans ferase; EC 2.3.1.6) activity was decreased significantly in the frontal neocortex and hippocampus 1 week and 4 months after surgery. The change in frontal neocortical S2 receptor site density (a) was inversely related to the level of choline acetyltransferase activity, (b) was specific for cholinergic denervation associated with the cortex but not the hippocampus, and (c) may represent a localized denervation supersensitivity due to degeneration of median raphe cortical afferents.